Looking to introduce a specific mutation into your gene of interest ?

Let our team create your custom mutant line.

Genome ProLab can help you introduce precise nucleotide changes into the Drosophila melanogaster genome. Whether you want to model a disease-associated variant, investigate a specific amino acid, or study a regulatory sequence, we tailor the strategy to your research objectives.

How Are Point Mutations Generated ?

CRISPR-Cas9 uses a guide RNA (gRNA) to direct Cas9 to a selected genomic sequence near the intended mutation. Cas9 creates a double-strand break, and a donor DNA template carrying the desired change is provided.

Through homology-directed repair (HDR), the cell can use this template to incorporate the mutation into the genome.

This approach enables defined nucleotide substitutions at the endogenous locus, allowing the modified gene to be studied in its native genomic context.

Precise Changes for Your Research

Amino acid substitutions

Introduce a specific amino acid change to investigate protein function, activity, stability, or interactions.

Disease-associated variants

Introduce changes corresponding to human disease-associated variants into a suitable Drosophila gene to investigate their biological effects.

Stop codons

Introduce a premature stop codon to investigate protein truncation or generate a potential loss-of-function allele.

Regulatory and splice-site mutations

Modify selected noncoding nucleotides to investigate their roles in gene regulation or RNA splicing.

A Strategy Tailored to Your Project

The editing strategy depends on the desired mutation, the surrounding DNA sequence, and the available genetic resources.

Selecting the target site

We assess suitable gRNAs close to the intended change. Existing gRNA resources may be considered when appropriate; otherwise, custom guides can be designed.

Designing the donor template

The donor carries the requested mutation and matching sequences surrounding the target region. Additional sequence changes may be proposed to prevent Cas9 from cutting the edited allele again. Any such changes are reviewed with you during project design.

Considering the genetic context

We consider the relevant transcript, the required genetic background, and the expected effect of the mutation. Alleles that cause lethality or sterility may require an appropriate maintenance strategy, such as the use of balancer stocks.

Planning multiple variants

If you want several mutations in the same gene, let us know whether they should be generated as separate alleles or combined in a single allele. Coordinating the designs may help reduce duplicated work.

Molecular Verification

The edited genomic region is verified by DNA sequencing to confirm the intended mutation and identify any additional changes within the sequenced region.

Molecular confirmation establishes the sequence of the allele; functional studies in your laboratory can then determine its biological effects.

Tell Us About Your Mutation

Provide your gene name or FlyBase ID, the desired nucleotide or amino acid change, and the relevant reference transcript or sequence accession. Please also indicate your preferred genetic background and any fly stocks or plasmids you already have.

For multiple changes, specify whether you need separate mutant lines or a combined mutant allele.

Our team will review your project and provide a proposed strategy and a customized quote.