WE KEEP IT SIMPLE

BUT SIGNIFICANT.

Custom Molecular Biology Services

From targeted mutations to custom plasmid construction

We provides reliable, high-quality molecular biology services for academic laboratories, research institutions, and biotechnology companies.

From a single targeted mutation to complex DNA assembly, our scientific team reviews each project and develops an appropriate cloning strategy to deliver a sequence-verified construct ready for your research.

Prices are in Canadian dollars and do not include taxes.


Targeted Mutagenesis

Precise. Verified. Ready to use.

Introduce substitutions, small insertions, or deletions into your plasmid without having to perform the cloning and screening yourself.

Template plasmid size (Kb)

Up to 6

6-8

8-10

10-12

*Obtain discount of 10 % for more than 10 plasmids to modify

+ $80 per additional mutation in the same template plasmid

Price ($)*

$160 / per mutation

$180 / per mutation

$200 / per mutation

$250 / per mutation

Project specifications

Modification

Maximum plasmid size

Customer-supplied vector

Plasmid Storage

Sequence verification

DNA delivered

Turnaround time

Any combination of substitutions, small insertions or deletions within a 10 bp region

12 kb

No additional vector preparation fee**

Free of charge for long-term storage and future projects

Full-length plasmid sequence/map provided

2–5 µg purified plasmid DNA

10–20 business days

**Customer-supplied plasmids must meet Genome ProLab submission requirements. See here.


Need something more complex?

Custom Plasmid Construction

From a simple subcloning project to a multi-fragment DNA assembly, Genome ProLab can design and execute the cloning strategy for your construct.

Because cloning difficulty can vary considerably depending on plasmid size, insert size, sequence complexity, number of fragments and the required cloning strategy, each custom project is scientifically evaluated before the final quotation is issued.

Our custom services

  • Gibson Assembly

    • Assembly of two or more DNA fragments into your plasmid of interest.

  • Subcloning

    • Transfer an insert, gene, regulatory sequence, tag, or other DNA element into a new vector.

  • PCR Cloning

    • PCR amplification and cloning of your sequence into the desired construct.

  • Large Insertions & Deletions

    • Introduce larger sequence modifications that fall outside our standard targeted mutagenesis service.

  • Gateway Cloning

    • Transfer sequences between Gateway-compatible entry and destination vectors.

  • Golden Gate Cloning

    • Seamless assembly of multiple DNA fragments into your desired vector using Type IIS restriction enzymes.

  • gRNA Cloning

    • Cloning of guide RNA sequences into compatible CRISPR/Cas vectors.

  • Gene Synthesis & Cloning

    • Have a DNA sequence synthesized and incorporated into the vector required for your project.

  • Custom Plasmid Construction

    • More complex plasmid modifications, including combinations of cloning strategies.

Custom project specifications

Project type

Typical price range

DNA delivered

Sequence verification

Turnaround time

Complex projects

Custom-designed according to your construct

Starting at $160 / construct or modification

2–5 µg purified plasmid DNA

Final full-length plasmid sequence/map provided

Typically 10–20 business days

Individual quotation